TABLE 5.

Transcriptional expression levels of 12 candidate differentially expressed genes between EF16-O2 and EF16^b

Gene identifier	Description or predicted function	Expression ratio (EF16-O2/ EF16) according to:
		RNA-Seq (P value)a	qRT-PCRc
OG1RF_RS11300	ABC transporter substrate-binding protein	2.48 (0.0013)	1.28 ± 0.17
OG1RF_RS11485	Ribose transporter RbsU	2.62 (0.0006)	1.40 ± 0.11
OG1RF_RS10660	ABC-F type ribosomal protection protein Lsa(A)	2.77 (0.011)	2.16 ± 0.14
OG1RF_RS06880	CoA-binding protein	3.37 (0.0003)	2.67 ± 0.35
OG1RF_RS12140	ABC transporter ATP-binding protein	3.75 (<0.0001)	15.70 ± 2.48
OG1RF_RS02205	ABC transporter ATP-binding protein	3.84 (<0.0001)	3.22 ± 0.49
OG1RF_RS05865	Sulfate ABC transporter ATP-binding protein	4.21 (<0.0001)	8.54 ± 1.24
OG1RF_RS06145	Molybdate ABC transporter substrate-binding protein	5.25 (<0.0001)	21.47 ± 4.53
OG1RF_RS09080	Sugar ABC transporter substrate-binding protein	5.40 (<0.0001)	39.61 ± 5.46
OG1RF_RS12590	ABC transporter permease	6.60 (<0.0001)	6.41 ± 0.79
OG1RF_RS00350	Alkaline shock response membrane anchor protein AmaP	6.72 (<0.0001)	2.23 ± 0.21
OG1RF_RS00630	BMP family ABC transporter substrate-binding protein	7.73 (<0.0001)	4.49 ± 0.64

^aThe DEGs of RNA sequencing were defined by a change ratio of ≥2 and a P value of <0.05.

^bAccording to RNA sequencing and qRT-PCR.

^cqRT-PCR data are given as means ± standard deviations of results from three independent experiments.