TABLE 6.
OMC MICs in E. faecalis derivatives with overexpressing candidate genes and their influence on population analysis profiling
| Transformed plasmid | Isolate | OMC MIC (mg/liter) for: |
PAP test results for: |
||||
|---|---|---|---|---|---|---|---|
| Parental straina | Vector control strainb | Derivative strainc | Parental strain | Vector control strain | Derivative strain | ||
| pRS00630 | EF16C2 | 0.5 | 0.5 | 0.5 | Negative | Negative | Positive |
| EF16C105 | 0.5 | 0.5 | 0.5 | Negative | Negative | Positive | |
| EF16C284 | 0.25 | 0.25 | 0.125 | Negative | Negative | Positive | |
| pRS12140 | EF16C105 | 0.5 | 0.5 | 0.5 | Negative | Negative | Positive |
| EF16C39 | 0.5 | 0.25 | 0.25 | Negative | Negative | Negative | |
| EF16C40 | 0.25 | 0.25 | 0.25 | Negative | Negative | Negative | |
| pRS02205 | EF16C2 | 0.5 | 0.5 | 0.5 | Negative | Negative | Negative |
| EF16C105 | 0.5 | 0.5 | 0.5 | Negative | Negative | Negative | |
| EF16C39 | 0.5 | 0.25 | 0.5 | Negative | Negative | Negative | |
| pRS06145 | EF16C2 | 0.5 | 0.5 | 0.5 | Negative | Negative | Negative |
| EF16C105 | 0.5 | 0.5 | 0.5 | Negative | Negative | Negative | |
| EF16C283 | 0.5 | 0.5 | 0.25 | Negative | Negative | Positive | |
| pRS06880 | EF16C2 | 0.5 | 0.5 | 0.5 | Negative | Negative | Negative |
| EF16C105 | 0.5 | 0.5 | 0.5 | Negative | Negative | Negative | |
| EF16C284 | 0.25 | 0.25 | 0.25 | Negative | Negative | Negative | |
| pRS11485 | EF16C2 | 0.5 | 0.25 | 0.25 | Negative | Negative | Negative |
| EF16C105 | 0.5 | 0.5 | 0.5 | Negative | Negative | Negative | |
| EF16C39 | 0.5 | 0.5 | 0.25 | Negative | Negative | Negative | |
a
The parental strain was an OMC-sensitive E. faecalis isolate without an OMC-heteroresistant phenotype according to the PAP test.
b
The vector control strain was the parental strain transformed with the pIB166 vector.
c
The derivative strain was the parental strain transformed with each overexpression plasmid.