TABLE 2.
Minimum duration for killing values for S. aureus Newman strains
| Strain | MDK90 ± SD (h)a |
||||||
|---|---|---|---|---|---|---|---|
| FLC | VAN | CFZb | CIP | DAP | RIF | SXT | |
| Wild type | 2.9 ± 0.2 | 2.4 ± 0.1 | 1.8 ± 0.1 | 0.6 ± 0.1 | 0.9 ± 0.1 | 2.2 ± 0.4 | 7.2 ± 0.7 |
| F128Y | 5.8 ± 0.3 (<0.0001) | 6.1 ± 1.0 (<0.0001) | 5.6 ± 1.7 (0.0013) | 1.5 ± 0.1 (<0.0001) | 2.4 ± 0.5 (0.0001) | 2.3 ± 0.3 (ns) | 7.1 ± 2.6 (ns) |
| F128Y comp | 2.8 ± 0.3 (ns) | 2.6 ± 0.1 (ns) | 2.0 ± 0.3 (ns) | 0.6 ± 0.1 (ns) | 1.3 ± 0.1 (ns) | 2.3 ± 0.1 (ns) | 7.1 ± 0.5 (ns) |
| L152F | 4.7 ± 0.8 (0.0015) | 4.6 ± 0.3 (0.0017) | 4.1 ± 0.5 (0.0329) | 0.9 ± 0.1 (0.0005) | 2.6 ± 0.1 (<0.0001) | 2.3 ± 0.3 (ns) | 6.1 ± 0.8 (ns) |
| L152F comp | 2.6 ± 0.1 (ns) | 2.3 ± 0.1 (ns) | 1.7 ± 0.3 (ns) | 0.6 ± 0.1 (ns) | 1.2 ± 0.2 (ns) | 2.7 ± 0.4 (ns) | 7.4 ± 1.5 (ns) |
a
MDK, minimum duration for killing. Values were determined from the triplicate data shown in Fig. 3 Abbreviations: Flucloxacillin (FLC), vancomycin (VAN), cefazolin (CFZ), ciprofloxacin (CIP), daptomycin (DAP), rifampin (RIF), trimethoprim-sulfamethoxazole (SXT), complemented (comp). Numbers in parentheses are multiplicity-adjusted P values determined by one-way ANOVA with Tukey’s multiple-comparison posttest. ns, P > 0.05.
b
Cefazolin did not achieve 90% killing against the mutants in the course of the experiment. Therefore, the MDK values shown are MDK50.