TABLE 3.
Area under the bacterial curve of CFZ and OXA treatment in standard and alternative mediaa
| Regimen | Medium or media | Mean AUBC ± SD |
|||
|---|---|---|---|---|---|
| 11-11 | MW2 | COL | BMC-1001 | ||
| CFZ | CAMHB-Tris (control) | 626.2 ± 6.6 | 658 ± 3.0 | 648.4 ± 2.8 | 633.9 ± 4.5 |
| CAMHB-Tris + 25 mM NAHCO3 | 455.3 ± 4.8b | 489.2 ± 2.5b | 612.1 ± 2.5 | 592.7 ± 3.4 | |
| CAMHB-Tris + 44 mM NAHCO3 | 310.0 ± 10.5b,c | 390.8 ± 4.8b,c | 601.9 ± 4.5 | 566.7 ± 2.9b | |
| RPMI 1640 + 10% LB medium | 378.3 ± 3.5b,c | 387.3 ± 6.9b,c | 505.3 ± 8.1b | 538.8 ± 6.3b | |
| OXA | CAMHB-Tris | 610 ± 3.5 | 661.9 ± 9.5 | 621.1 ± 3.6 | 642.4 ± 5.1 |
| CAMHB-Tris + 25 mM NAHCO3 | 480 ± 6.3b | 539.2 ± 23.3b | 596.7 ± 6.7 | 617.3 ± 2.6 | |
| CAMHB-Tris + 44 mM NAHCO3 | 392 ± 17.8b,c | 399.4 ± 15.0b,c | 568.9 ± 11.0b | 603.0 ± 538 | |
| RPMI 1640 + 10% LB medium | 415.1 ± 4.2b,c | 438.1 ± 9.3b,c | 649.7 ± 4.7 | 599.1 ± 9.4 | |
a
The area under the bacterial curve (AUBC) is inversely related to antibiotic activity, with lower AUBC indicating greater antibiotic effect. The data represent means ± the standard deviations of duplicate replicates, with two samples taken at each time point (n = 4). CAMHB-Tris includes 2% NaCl.
b
P < 0.05 versus control.
c
P < 0.05 versus 25 mM NAHCO3.