Table 1.
Primary antibodies | Clone | Isotype | Working dilution | Supplier |
---|---|---|---|---|
Anti-CD163 mAb | 2A10/11 | IgG1 | 1:200 | Bio-rad |
Anti-CD163 pAb | Polyclonal | IgG | 1:200 | R&D Systems |
Anti-Sn mAb | 41D3 | IgG1 | 1:50 | [27] |
Anti-Sn mAb | 26B2 | IgG2b | 1:30 | [29] |
Anti-MHCII mAb | MSA3 | IgG2a | 1:200 | Kingfisher Biotech |
Anti-CD14 mAb | MIL2 | IgG2b | 1:100 | [28] |
Anti-CD1c mAb | L161 | IgG1 | 1:50 | Biolegend |
Anti-cytokeratin mAb | AE1/AE3 | IgG1 | 1:50 | Dako |
Anti-vimentin mAb | V9 | IgG1 | 1:50 | Bio-rad |
Anti-PRRSV nucleocapsid protein mAb | 13E2 | IgG2a | 1:50 | [32] |
Anti-PCV2 Cap mAb | 12E12 | IgG2a | 1:50 | [30] |
Anti-PRV gB mAb | 1C11 | IgG2b | 1:100 | [31] |
Anti-PRV gD mAb | 13D12 | IgG1 | 1:50 | [31] |
mAb: monoclonal antibody, pAb: polyclonal antibody.