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. 2020 Feb 24;51:21. doi: 10.1186/s13567-020-00751-7

Table 1.

Antibodies used for immunofluorescence staining and flow cytometry

Primary antibodies Clone Isotype Working dilution Supplier
Anti-CD163 mAb 2A10/11 IgG1 1:200 Bio-rad
Anti-CD163 pAb Polyclonal IgG 1:200 R&D Systems
Anti-Sn mAb 41D3 IgG1 1:50 [27]
Anti-Sn mAb 26B2 IgG2b 1:30 [29]
Anti-MHCII mAb MSA3 IgG2a 1:200 Kingfisher Biotech
Anti-CD14 mAb MIL2 IgG2b 1:100 [28]
Anti-CD1c mAb L161 IgG1 1:50 Biolegend
Anti-cytokeratin mAb AE1/AE3 IgG1 1:50 Dako
Anti-vimentin mAb V9 IgG1 1:50 Bio-rad
Anti-PRRSV nucleocapsid protein mAb 13E2 IgG2a 1:50 [32]
Anti-PCV2 Cap mAb 12E12 IgG2a 1:50 [30]
Anti-PRV gB mAb 1C11 IgG2b 1:100 [31]
Anti-PRV gD mAb 13D12 IgG1 1:50 [31]

mAb: monoclonal antibody, pAb: polyclonal antibody.