Skip to main content
. 2020 Feb 12;32:101464. doi: 10.1016/j.redox.2020.101464

Fig. 6.

Fig. 6

Omeprazole increased the BclxL/Bax ratio and autophagy in HK-2 cells. A) HK-2 cells were stimulated with 300 μM omeprazole and Bax and BclxL protein expression was assessed by Western blot. Representative Bax and BclxL Western blot. Mean ± SD of three of four independent experiments. *p < 0.05 vs control. B) BclxL/Bax ratio. Mean ± SD of three independent experiments. *p < 0.05 vs control. C) Cells were pretreated with BclxL-BH4 peptide 1 h before omeprazole stimulation, and cell viability was assessed by MTT. Mean ± SD of three independent experiments. **p < 0.05 vs control. D) Representative Western blot and quantification of LC3II/LC3I ratio in HK-2 cells pretreated with different doses of 3-MA for 1h and stimulated with 300 μM omeprazole for 24 or 48h. Mean ± SD of three independent experiments. *p < 0.05 vs control; #p < 0.05 vs 3-MA 0 μM. E) Cell viability and (F) cytotoxicity of HK-2 cells pretreated with different doses of 3-MA for 1 h and stimulated with 300 μM omeprazole for 48 h. Mean ± SD of four or three independent experiments. ***p < 0.001 vs control.