Figure 2.

poSn interacts with DAP12 in PRRSV-infected PAMs.A PAMs were infected with rBJ4-EGFP (MOI = 1) for 24 h. Cells were fixed by 4% PFA for 15 min at RT and permeabilized with 0.2% Tween-20 for membrane protein staining. poSn was stained with DyLight 405 (blue) and DAP12 was stained with DyLight 649 (red). The co-localization of the two proteins was visualized by confocal microscopy. Scale bars, 10 μm. B HEK-293T cells were co-transfected with poSn-myc-his (10 μg) and 3×Flag-DAP12 (6 μg) for 48 h. WCLs were subjected to IP assays with anti-myc mAb or anti-Flag mAb. IB was performed to detect the indicated proteins. The confocal images were representative from two independent experiments, and IB panels were representative from three independent experiments.