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. 2020 Feb 24;51:18. doi: 10.1186/s13567-020-00743-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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poSn-DAP12 pathway mediates the inhibition of type I IFN production in response to poly (I:C).A PAMs were transfected with sipoSn-332# or sipoSn-1983# for 36 h, and then stimulated with 0.25 μg/mL poly (I:C) for 12 h. RT-qPCR analysis was performed to measure the mRNA abundance of poSn and IFN-α/β. B, C CRL-2843-CD163 cells with poSn overexpression were transfected with 2.5 μg/mL poly (I:C) for the indicated time periods (0, 2, 4 h). poSn expression was determined by IB (B). IFN-β transcription was examined by RT-qPCR (C). D, EDAP12 knockdown PAMs were stimulated with 2 μg/mL poly (I:C) for the indicated time periods (0, 3, 6 h). DAP12 knockdown was determined by IB (D). IFN-α/β transcription was detected by RT-qPCR (E). Quantification data were indicated as mean ± SEM. Statistical significances were shown by Student t test: *p < 0.05, **p <0.01, ***p <0.001.