Table 1.
Primers for RT-qPCR and plasmid construction
| Primers | Sequence (5′–3′) |
|---|---|
| pig GAPDH-Forward | CCTTCCGTGTCCCTACTGCCAAC |
| pig GAPDH-Reverse | GACGCCTGCTTCACCACCTTCT |
| pig IFN-β-Forward | TGCAACCACCACAATTCC |
| pig IFN-β-Reverse | CTGAGAATGCCGAAGATCTG |
| pig IFN-α-Forward | GCCTCCTGCACCAGTTCTACA |
| pig IFN-α-Reverse | TGCATGACACAGGCTTCCA |
| poSn-Forward | CGTGTTGTGGCCTCTTCTCT |
| poSn-Reverse | CACGTTGCAAGTCAGGTTGG |
| pig DAP12-Forward | ACCCGGAAACAACACATCGC |
| pig DAP12-Reverse | TACTGCCTCTGTGTGTTGAGG |
| PRRSV-ORF7-Forward | AAACCAGTCCAGAGGCAAGG |
| PRRSV-ORF7-Reverse | GCAAACTAAACTCCACAGTGTAA |
| poSn-infusion-Forward | CTGGCTAGTTAAGCTATGGACTTCCTGCTCCTGCT |
| poSn-infusion-Reverse | GCCCTCTAGACTCGAGGACTGTGCTTTTCACAGA |
| poSn-ECD-infusion-Reverse | GCCCTCTAGACTCGAGGTGCTGGAACAGATGCA |
| poSn-TCD-Forward | CCAAGCTTGCCACCATGCTTCTCTGGTTCCT |
| poSn-TCD-Reverse | CCGCTCGAGGACTGTGCTTTTCACAGACTG |
| DAP12-Forward | CCCAAGCTTGCCACCATGGGAAGACTGGGGCCAT |
| DAP12-XhoI-Reverse | CCGCTCGAGTCACTTGTAGTACTGCCGCTGGGTA |
| DAP12-ΔICD-Forward | CGGAATTCTCAGAGAGAATGCAGCTGCT |
| DAP12-ΔICD-Reverse | GCTCTAGATCACACAGCCAGGGCGAT |
| DAP12-ΔECD-Forward | CGGAATTCTGGCATCCTGGCG |
| DAP12-ΔECD-Reverse | GCTCTAGATTTGTAATACTGCCTCTGTGTGTTG |
| DAP12(D50A)-Forward | GGGGATCTGGTGCTGGCCCTCCTCATCGCCCTG |
| DAP12(D50A)-Reverse | CAGGGCGATGAGGAGGGCCAGCACCAGATCCCC |
| DAP12-ΔTM1-Forward | GCTGCTCCGCCGTGAGCCCCCTGGGGGATCTGGTGCTGAC |
| DAP12-ΔTM1-Reverse | GTCAGCACCAGATCCCCCAGGGGGCTCACGGCGGAGCAGC |
| DAP12-ΔTM2-Forward | GCATCCTGGCGGGGATCGTGCTCCTCATCGCCCTGGCTGT |
| DAP12-ΔTM2-Reverse | ACAGCCAGGGCGATGAGGAGCACGATCCCCGCCAGGATGC |
| DAP12-ΔTM3-Forward | TGGGGGATCTGGTGCTGACCTACTCCCTGGGTCGGCTGGT |
| DAP12-ΔTM3-Reverse | ACCAGCCGACCCAGGGAGTAGGTCAGCACCAGATCCCCCA |