Fig. 3.

TRAF1-deficiency promotes neutrophil recruitment. a. OCT-embedded back skin sections from Traf1^−/− and WT mice infected with C. albicans (1 × 10⁷ CFU) were stained by Ly6G antibody (red) and DAPI (blue). The Ly6G⁺ Cells in the skin tissues from Traf1^−/− (n = 8) and WT mice (n = 7) were quantified by the average of positive cells from 5 objective fields (400×) each mouse. PBS group, the control group; CA group, the C. albicans infection group. b. Traf1^−/− mice (n = 3) and WT mice (n = 3) were treated with 40 μg of Ly6G mAb or control IgG via intraperitoneal injection (i.p.) 2 h before C. albicans (1 × 10⁷ CFU) infection. The Skin tissues were harvested on day 3 after infection for immunofluorescence and PAS staining. c. OCT-embedded back skin sections from Traf1^−/− and WT mice pretreated wtih Ly6G mAb or IgG were stained by Ly6G antibody (red) and DAPI (blue), and the Ly6G⁺ cells in the skin tissues were quantified by an average of positive cells from 3 objective fields (200×) each mouse. d. OCT-embedded skin sections from Traf1^−/− mice and WT mice pretreated with Ly6G mAb or IgG were stained by PAS. Representative micrographs were captured at 50× magnification. Data are shown as mean ± SEM, and were analyzed using the unpaired, two-tailed, Student’s t-test. Values of p below 0.05 represented a statistically significant difference