Table 2.

Summary of the main effects induced by single chemicals administered at IC₂₀, IC₅ and IC₂₀/100. In bracket, the lowest concentration eliciting a statistically significant modification of at least one of the measured DNT features is indicated (Phase 2)

3 days	BPA	CPF	Lead	Methyl-Hg	PCB138	VA
Synaptogenesis (SYP, PSD95)	⇓ PSD95t (IC20)	⇑ SYPt (IC20)	⇓ PSD95n (IC20/100)	⇔	⇑ PSD95n (IC20)	⇔
Neurite outgrowth	⇔⇑ n. neurites (IC5)	⇓ ⇔	⇔	⇔	⇑ (IC20/100)	⇑ n. neurites (not shown) (IC5)
BDNF levels	⇔	⇑ BDNFt (IC20)	⇔	⇔	⇔ ⇑ ratio (IC20/100)	⇔
14 days	BPA	CPF	Lead	Methyl-Hg	PCB138	VA
Synaptogenesis (SYP, PSD95)	⇓ PSD95n (IC5)	⇓ SYPn ⇑ PSD95t (IC5)	⇑ SYPt ⇓ PSD95n (IC20/100)	⇓ SYPn (IC5)	⇔	⇔
Neurite outgrowth	⇓ n. branch points (IC5)	⇓ ⇔	⇔	⇓ n. branch points (IC20)	⇔	⇔
BDNF levels	⇔ ⇓ ratio (IC5)	⇑ BDNFt (IC20) ⇓ ratio (IC20)	⇔	⇓ ratio (IC20)	⇑ (IC5)	⇑ BDNFn and ratio (IC20)

Tested concentrations: BPA (0.29 μM, IC₂₀/100; 12.74 μM, IC₅; 28.96 μM, IC₂₀); CPF (0.37 μM, IC₂₀/100; 21.01 μM, IC₅; 37.10 μM, IC₂₀); Lead (0.0073 μM, IC₂₀/100; 0.17 μM, IC₅; 0.73 μM, IC₂₀); Methyl-Hg (0.0013 μM, IC₂₀/100; 0.05 μM, IC₅; 0.13 μM, IC₂₀); PCB138 (0.0593 μM, IC₂₀/100; 3.53 μM, IC₅; 5.93 μM, IC₂₀); VA (2.1 μM, IC₂₀/100; 70 μM, IC₅; 210 μM, IC₂₀)