Table 4.

Main effects and mixture-related LOAECs specific for each time interval and DNT endpoint

3 days	‘3-Sim’ Similar MoA	‘3-Diss’ Dissimilar MoA	‘All’
Synaptogenesis (SYP, PSD95)	⇑ SYPt ⇑ synapses (LOAEC/4-syn) BPA, CPF	⇑ SYPt ⇑ synapses (LOAEC/4-syn) Methyl-Hg, PCB138	⇑ SYPt (LOAEC/4-syn) BPA, CPF, Methyl-Hg, PCB138
Neurite outgrowth and % of neurons	⇓ n. branch points ⇓ neurite length (LOAEC/4-neu) ⇑ β-III-tubulin+ neurons (LOAEC/2-neu) CPF	⇓ n. neurites/neuron (LOAEC/4-neu) PCB138 (counteract)	⇓ n. neurites/neuron (LOAEC/4-neu) ⇑ β-III-tubulin+ neurons (LOAEC-neu) CPF PCB138 (counteract)
BDNF levels	⇑ total BDNF (LOAEC-bdnf)	⇑ total BDNF (LOAEC-bdnf)	⇑ total BDNF (LOAEC/2-bdnf)
14 days	‘3-Sim’ Similar MoA	‘3-Diss’ Dissimilar MoA	‘All’
Synaptogenesis (SYP, PSD95)	⇑ SYPt (LOAEC/2-syn) BPA	⇔	⇑ SYPt (LOAEC-syn) BPA
Neurite outgrowth and % of neurons	⇓ neurite length (LOAEC/4-neu) ⇑ β-III-tubulin+ neurons (LOAEC/2-neu) CPF, Lead	⇓ n. branch points ⇓ neurite length ⇑ β-III-tubulin+ neurons (LOAEC/2-neu) Methyl-Hg, PCB138, VA (counteract)	⇓ n. branch points ⇓ neurite length ⇑ β-III-tubulin+ neurons (LOAEC/2-neu) CPF, Lead, Methyl-Hg, PCB138 VA (counteract)
BDNF levels	⇑ total BDNF (LOAEC/2-bdnf) CPF, Lead	⇔ ⇑ total BDNF (LOAEC/2-bdnf) Methyl-Hg	⇑ total BDNF (LOAEC/2-bdnf) CPF, Lead, Methyl-Hg