Fig. 3.

Pupation site choice behavior for Drosophila melanogaster, D. simulans, and their F1 hybrids. The proportion of individuals that pupated on the food surface for males and females from both species and their F1 hybrids. D. melanogaster males and females were taken from the LH_M strain, whereas D. simulans males and females were taken from the Lhr strain. F1 hybrids resulted from a cross between these two strains. The “melX” hybrid males have the D. melanogaster X chromosome, and the “simX” hybrid males have the D. simulans X chromosome. Both hybrids have D. melanogaster cytoplasmic inheritance. Box plots display the median (bold bar), interquartile range (box), and full extent of the data set excluding outliers (whiskers). Those labeled with different letters are significantly different from one another based on pairwise Wilcoxon tests followed by sequential Bonferroni correction for multiple comparisons (all P < 0.0001 after correction, N = 33 for D. melanogaster and melX/F1 females, N = 36 for simX, and N = 31 for D. simulans).