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. Author manuscript; available in PMC: 2020 Feb 24.

Published in final edited form as: Colloids Surf B Biointerfaces. 2014 Jun 11;121:141–149. doi: 10.1016/j.colsurfb.2014.06.011

Fig. 3. — Fluorescent imaging analysis (a) and spectrophotometric analysis (b) of the cellular uptake and internalization of immunocerasomes in comparison to the cerasome control. A431, DU145, and HL-60 cells were incubated with immunocerasomes or cerasomes at a lipid concentration of 185 μM for 3 hr. (a) The cells were fixed and stained against nucleus by DAPI (blue), while immunocerasomes and cerasomes were labeled using NBD-DPPE (green). Scale bar: 25 μm (b) The relative amount of internalized immunocerasomes or cerasomes was measured by the NBD-DPPE fluorescence intensity of the cell lysates. The averages and errors were calculated from measurement of three replicate specimens. The p values were calculated to be 0.00059 for the endocytosis of cerasomes vs. immunocerasomes in A431 cells, 0.00031 in DU145 cells, and 0.117 in HL-60 cells, respectively.

Fig. 3. — Fluorescent imaging analysis (a) and spectrophotometric analysis (b) of the cellular uptake and internalization of immunocerasomes in comparison to the cerasome control. A431, DU145, and HL-60 cells were incubated with immunocerasomes or cerasomes at a lipid concentration of 185 μM for 3 hr. (a) The cells were fixed and stained against nucleus by DAPI (blue), while immunocerasomes and cerasomes were labeled using NBD-DPPE (green). Scale bar: 25 μm (b) The relative amount of internalized immunocerasomes or cerasomes was measured by the NBD-DPPE fluorescence intensity of the cell lysates. The averages and errors were calculated from measurement of three replicate specimens. The p values were calculated to be 0.00059 for the endocytosis of cerasomes vs. immunocerasomes in A431 cells, 0.00031 in DU145 cells, and 0.117 in HL-60 cells, respectively.