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. Author manuscript; available in PMC: 2021 Feb 20.
Published in final edited form as: Cell Chem Biol. 2019 Dec 23;27(2):232–244.e7. doi: 10.1016/j.chembiol.2019.12.004

Figure 4. E07-Aptamer Loaded Magnetic Beads + A Matched Antidote Reversibly Bind and Isolate a Pure Population of EGFR(+) Cells Out of Blood.

Figure 4.

(A–D) Overview of E07 aptamer-antidote MACS purification of EGFR(+) cells out of blood. (A) A431 cells were spiked into human blood and red blood cells lysed prior to the addition of biotinylated-E07 attached to SA-magnetic beads. (B) MACS purification was used to specifically isolate E07-bead bound cells, allowing unbound cells to be washed away. (C) Subsequent treatment with 100-fold excess antidote mA9 stripped the E07-magnetic beads from isolated cells, and (D) additional magnetic-based pull down of the aptamer-antidote neutralized beads left a pure population of isolated cells in the supernatant. € EGFR(+) A431 cells were labeled with DiI dye, and dye incorporation verified by flow cytometry. (F) Blood gave a SSC vs. FSC profile unique from that of the EGFR(+) A431 cells and was negative for the DiI dye. (G) Treatment with E07-beads plus the specific mA9 antidote recovered a ~95% pure population of DiI-labeled EGFR(+) cells, as evidenced by flow cytometry analysis of recovered cells. See also Table S2 and Figure S4.