Figure 5.

Identification and profiling of chimeric RNA candidates. Sanger sequencing of chimeric RNA candidates from read-through (A), intra-others (B) and inter-chromosomal (C). The chimeric RNA expression in human normal tissues was examined by qRT-PCR and followed by gel electrophoresis (D). Tissue specific fusion RNA, TMED6-COG6, was found only in liver, colon and stomach by qRT-PCR, whereas the wild-type parental gene TMED6 was found in a different list of tissues (E and F). GAPDH was used as internal control.