Figure 1.

Human NER factors XPA and RPA. (A) Schematic diagram of the NER pre-incision complex. DNA damage is marked with a star on the substrate. (B) Schematic diagram of the domain structure of human XPA. The XPA DNA binding region, which contains the globular core, is depicted as an oval. DNA- and RPA-binding residues are indicated below the domain, aligned with the XPA residues involved in each interaction. Both previously reported sites for RPA70AB interaction with the XPA globular core are marked for clarity. (C) Schematic diagram of the subunit and domain structure of human RPA heterotrimer. Globular domains are depicted as ovals. All globular domains are OB-folds except for the winged-helix domain RPA32C. (D) Schematic representation of the spatial organization of RPA domains when bound to ssDNA. The RPA DNA binding apparatus consists of RPA70A, RPA70B, RPA70C, RPA32D, and RPA14. A ssDNA molecule of ∼40 nts is depicted as a ribbon. The XPA-binding regions are labeled with arcs.