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Optimization of RNase H reaction conditions. (A) Proportion of rRNA-aligning reads of total mapped reads (% rRNA reads), for un-depleted and RNase H depleted samples for two RNase H enzymes and various reaction times with probe-to-RNA fixed to 1:1, or (B) various probe-to-RNA ratios with reaction time fixed to 30 min. The same RNA sample isolated from Bacteroides dorei was split for rRNA depletion across different reaction conditions.
