Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 24;48(4):1779–1789. doi: 10.1093/nar/gkaa005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — The extent of tissue-specific and individual-specific ABS events in human fetal and adult tissue transcriptomes. (A) A schematic diagram of the PCU metric for the predominant circRNA. Colored boxes, exons. Black lines, introns. Gray polylines, canonical splicing. Colored arcs, back-splicing. Circles, circRNAs. (B) The fraction of ABS events in fetal tissues with the same predominant circRNA for A5BS events and A3BS events separately. (C) The PCUs in adult human uterus for an A5BS event in the HIPK3 gene (left panel) and an A3BS event in the EXOSC1 gene (right panel) calculated using RNA-seq data in two tissue samples and measured using RT-qPCR in one tissue sample, respectively. (D) The distribution of PCUs for A5BS events (left panel) and A3BS events (right panel) across human fetal tissues. (E) Visualization of A5BS events produced from the MCF2L2 gene across human fetal tissues. The A5BS events were sufficiently abundant for their PCUs to be calculated in 9 of 20 fetal tissues. The two A5BS circRNAs are indicated by dark red and light red arcs with raw back-splice junction reads (average RPM of two biological replicates) above the arcs. The PCU of the ABS event in each tissue is provided to the right. Colored arcs, back-splicing. (F) The fraction of tissue-specific A5BS events (left panel) and A3BS events (right panel) among human fetal tissues with ΔPCU ranging from 10 to 40. (G) Spearman correlations of PCUs for ABS events, circRNAs, and their host genes in the same tissue among different individuals and in the same individual among different tissues, respectively.