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. 2019 Dec 12;48(4):e19. doi: 10.1093/nar/gkz1165

Figure 3.

Figure 3.

TtAgo cleaves more specifically with short guides. (A) Cleavage efficiencies as functions of guide length at 70°C (top) and 75°C (bottom). TtAgo in complex with the MP12 guide was used to target either KRAS (S) WT or G12D. (B) Cleavage efficiencies of (top) DNA (S) and (bottom) RNA (S) as functions of temperature with 19 nt long guide (KRAS-S (19 nt)-MP12). (C) Cleavage efficiencies of DNA (S) and RNA (S) as functions of temperature with 16 nt long guide (KRAS-S (16 nt)-MP12). Experiments were carried out in Buffer 3 with TtAgo: guide: target ratio 5:1:1. Incubation time 1 h. N = 3.