Table 2.
Summary of the synthesized m7GpppNpG trinucleotide cap analogues
| Sequence | pNpG [μmol] | m7GDP-Im [μmol] | ZnCl2 [μmol] | m7GpppNpG [μmol] | Yield | m/zcalcd. | m/zfound |
|---|---|---|---|---|---|---|---|
| m7GpppApG | 19.6 | 39.1 | 391 | 12.4 | 64% | 1130.13266 | 1130.13406 |
| m7GpppAmpG | 16.5 | 33.0 | 330 | 10.2 | 62% | 1144.14831 | 1144.14978 |
| m7Gppp(m6A)pG | 7.56 | 18.9 | 151 | 5.00 | 66% | 571.57052a | 571.56962a |
| m7Gppp(m6Am)pG | 15.2 | 30.4 | 304 | 10.2 | 67% | 1158.16396 | 1158.16495 |
| m7GpppCpG | 8.51 | 43.4 | 349 | 2.28 | 27% | 1106.12142 | 1106.12244 |
| m7GpppCmpG | 5.22 | 27.0 | 190 | 2.49 | 48% | 1120.13707 | 1120.13812 |
| m7GpppGpG | 17.3 | 52.5 | 180 | 8.0 | 46% | 1146.12757 | 1146.12867 |
| m7GpppGmpG | 17.5 | 35.0 | 350 | 8.6 | 49% | 1160.14322 | 1160.14446 |
| m7GpppUpG | 3.35 | 16.8 | 171 | 0.86 | 26% | 1107.10544 | 1107.10683 |
| m7GpppUmpG | 4.76 | 25.6 | 211 | 2.26 | 47% | 1121.12109 | 1121.12242 |
a m/z of [M–2H]2– ion.