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. Author manuscript; available in PMC: 2020 Feb 24.
Published in final edited form as: Bioorg Med Chem. 2013 Mar 14;21(12):3523–3532. doi: 10.1016/j.bmc.2013.03.001

Figure 5.

Figure 5.

Live cell FRET study of the in vivo interaction between the C-terminal TC-tagged Vif and Alexa fluor 594 tagged A3G 211–225. (A) Donor and acceptor fluorescent confocal micrographs of transfected cells excited with a 509 nm laser excitation before the acceptor bleaching scan are overlaid in an [r, g, b] format. Inset in (A) shows the emission intensity measured during the bleaching scan, indicating the bleached ROI has a significant acceptor concentration. (B) The same overlaid images after the acceptor-bleaching scan. (C) Donor and acceptor fluorescent confocal micrographs of non-transfected cells excited with a 509 nm laser excitation before the acceptor bleaching scan are overlaid in an [r, g, b] format. Inset in (C) shows the emission intensity measured during the bleaching scan, indicating the bleached ROI has a significant acceptor concentration. (D) The same overlaid images after the acceptor-bleaching scan. (E) FRET efficiency versus acceptor/donor ratio intensity ratio.