Figure 3.

HK2 is a direct target of miR-202. (A) HK2 3′-UTR sequences containing the wild-type or mutated binding site of miR-202 were cloned into the psiCHECK2 vectors. (B) mRNA expression levels of HK2 following 97-L and Huh7 cell transfection with miR-NC or miR-202 mimic. (C) Protein expression levels of HK2 following 97-L and Huh7 cell transfection with miR-NC or miR-202 mimic. (D) Transfection of miR-202 mimic in 97 L cells markedly suppressed the luciferase activity of HK2 3′-UTR wild-type reporter construct, but not the mutant HK2 3′-UTR construct. Data are presented as the mean ± SD from three independent experiments. *P<0.05. HK2, hexokinase 2; 3′UTR, 3′untranslated region; WT, wild-type; Mut, mutant; NC, negative control.