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. 2020 Feb 17;11:46. doi: 10.3389/fendo.2020.00046

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Copyright © 2020 Feng, Xu, Pan, Hu, Wu, Lin, Zhang, Ji, Hu, Zhong, Yan, Zhong and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The function of BAT is dramatically reduced in 10-months-old mice. H&E staining (A) and immunohistochemistry analysis against UCP1 (B) in interscapular BAT from 6- to 8-week-old and 10-months-old mice. (C,D) qPCR analysis of the thermogenesis and senescence associated genes in interscapular BAT. (E,F) Western blot for UCP1 and GAPDH in BAT of young and old groups. Data are presented as the mean ± standard deviation (S.D.). Statistical significance evaluated by unpaired Student's t-test is reported as **p < 0.01 and ***p < 0.001.