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. 2020 Feb 17;11:46. doi: 10.3389/fendo.2020.00046

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Copyright © 2020 Feng, Xu, Pan, Hu, Wu, Lin, Zhang, Ji, Hu, Zhong, Yan, Zhong and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NONMMUT024512 and n281160 may involve in regulation of Pparα expression. (A) Representative microscopic images at day 0 and 4 of differentiation, and Oil red O staining at day 8. (B) UCP-1 immunofluorescence of primary brown adipocytes at day 8 of differentiation (original magnification, ×200). (C) Transcriptional levels of Ucp1, Pparα and two candidate lncRNAs at indicated times during the differentiation of brown adipocytes. (D–G) The expression of Pparα in adipocytes transfected with siRNAs targeting NONMMUT024512 or n281160 at day 6 of differentiation. Data are presented as the mean ± standard deviation (S.D.). Statistical significance evaluated by unpaired Student's t-test is reported as *p < 0.05, **p < 0.01, and ***p < 0.001.