Diverse, in-frame, IgHV-TCRDJC rearrangements are expressed in thymus and periphery of nurse shark. Amino acid translation showing CDR3 regions amplified from IgHV forward and TCRδ reverse primers from (A) thymus, (B) spleen, and (C) spiral valve. Clone name is to the left of alignment, followed by the IgH V segment origin, with the TCRδJ used to the right of the alignment. Sequences are aligned to the conserved cysteine of the YxC motif of the V domain and the FGxG motif of the J. Nucleotides within CDR3 are aligned in the center, and conserved residues of the genomic D segment are highlighted by frame (first frame, cyan; second frame, green; third frame, yellow) and flanked by nontemplate and palindromic residues. Asterisks (*) denote stop codons, and slashes (/) denote frameshifts.