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. 2020 Jan 16;19(3):2475–2480. doi: 10.3892/ol.2020.11312

Figure 3.

Figure 3.

Overexpression of ELF3-AS1 in osteosarcoma cells upregulates KLF12 potentially via downregulation of miR-205. (A) Expression levels of ELF3-AS1, KLF12 and miR-205 in cells transfected with ELF3-AS1, KLF12 expression vectors or miR-205 mimic. (B) miR-205 expression in cells with ELF3-AS1 overexpression (left), and ELF3-AS1 expression in cells with miR-205 overexpression (right). (C) KLF12 expression at mRNA and protein levels in cells with ELF3-AS1 and/or miR-205 overexpression. (D) ELF3-AS1 (left) and miR-205 (right) expression in cells with KLF12 overexpression. *P<0.05. C, untransfected control; NC1, empty vector negative control; NC2, miRNA negative control; KLF12, Kruppel-like factor 12; miR, microRNA; ELF3-AS1, ELF3-antisense RNA 1.