Fig. 1.

Cysteamine protects primary cortical neurons from mHtt toxicity. Primary cortical neurons were transfected at 7 DIV with either Htt N586 82Q or Htt N586 22Q and treated with different doses of cysteamine. After 48 h, neurons were fixed and stained with DAPI and MAP2 was immunodetected. A) Representative pictures of transfected neurons. Scale bar: 25 μm. B) mHtt toxicity measured by nuclear condensation assay was reduced by cysteamine, Results presented as means ± S.E. of the percentage of dead cells. *p < 0.05 vs Htt N586 82Q by ANOVA with Bonferroni post-hoc test. (n = 4 independent neuronal preparations).