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. Author manuscript; available in PMC: 2020 Feb 24.
Published in final edited form as: Methods Mol Biol. 2019;1957:107–120. doi: 10.1007/978-1-4939-9158-7_7

Fig. 2.

Fig. 2

Schematic overview of the in-cell assay. (a) Transfect the cells at 90–100% confluency with HA-ASK1, HA-JNK3, and the appropriate Venus-arrestin construct. (b) Allow 48 h for protein expression with serum starvation overnight before the assay. The cells can be monitored using a fluorescent microscope. Cells that have been transfected will glow green because of the Venus (GFP) transfection. (c) Lyse the cells to release the protein using the lysis buffer described below. (d) Run a Western blot for arrestin expression (GFP), ASK1 and JNK3 expression (HA), and ppJNK