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. 2019 Dec 24;219(1):e201904203. doi: 10.1083/jcb.201904203

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© 2019 Park et al.

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Figure 1. — WBP11 is required for centriole duplication. (A) Immunoblot showing a time course of siRNA-mediated depletion of WBP11. (B) Quantification of centriole number in mitotic cells 72 h after siRNA-mediated depletion of either STIL or WBP11. n = 3, ≥49 cells per experiment. Error bars represent SD. (C) Quantification of centriole number in mitotic cells 72 h after depletion of WBP11 with one of four independent siRNAs. n = 3, ≥47 cells per experiment. Error bars represent SD. (D) Immunoblot showing coimmunoprecipitation (IP) of endogenous PP1 with WBP11^WT-EYFP, but not WBP11^ΔPP1-EYFP. (E) Immunoblot showing expression levels of WBP11-EYFP transgenes 72 h after transfection with a WBP11 siRNA. Cells were induced to express the WBP11-EYFP transgenes with doxycycline. (F) Quantification of centriole number in mitotic cells 72 h after siRNA-mediated knockdown of WBP11. Cells were induced to express an RNAi-resistant WBP11 transgene with doxycycline. n = 4, ≥47 cells per experiment. Error bars represent SD. (G) Representative images of cells from F expressing an RNAi-resistant WBP11^WT-EYFP transgene. Scale bars represent 5 µm; 1 µm in zoomed-in region. (H) Representative images of cells from F expressing an RNAi-resistant, WBP11^ΔPP1-EYFP transgene. Scale bars represent 5 µm; 1 µm in zoomed-in region.