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. 2019 Dec 24;219(1):e201904203. doi: 10.1083/jcb.201904203

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© 2019 Park et al.

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Figure 6. — WBP11 and SNW1 are required for proper splicing of the TUBGCP6 pre-mRNA. (A) Schematic of the TUBGCP6 gene. The 3′ end of the gene is expanded and aligned to Integrative Genomics Viewer (IGV) reads to highlight retained introns (*) observed after loss of WBP11 or SNW1. (B) RT-PCR analysis of splicing of TUBGCP6 intron 21 following depletion of WBP11 or SNW1. Schematic shows the position of the primers used for PCR and the expected product size. (C) Immunoblot showing TUBGCP6 expression levels over a 3-d period following WBP11 or SNW1 siRNA knockdown. (D) Immunoblot showing TUBGCP6 expression levels in WBP11^WT or WBP11^AID cells after 1 or 2 d of auxin addition.