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. 2019 Dec 24;219(1):e201904203. doi: 10.1083/jcb.201904203

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© 2019 Park et al.

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Figure 8. — Expression of intronless TUBGCP6 can partially rescue centriole duplication failure after WBP11 depletion. (A) Immunoblot showing TUBGCP6 levels after 48 h of siRNA-mediated depletion of SNW1 or WBP11. Cells on the right expressed the FLAG-TUBGCP6 transgene. (B) Quantification of centriole number in mitotic cells after siRNA-mediated knockdown of SNW1 or WBP11 for 48 h. FLAG-TUBGCP6 transgene was induced with doxycycline where indicated. n = 3 (SNW1 and STIL), n = 4 (control and WBP11), ≥50 cells per experiment. Error bars represent SD. (C) Graph showing the percentage of mitotic cells with three to four centrioles, as shown in Fig. 8 B. Unpaired parametric t test: *, P = 0.0343 (WBP11) and 0.0353 (SNW1). Error bars represent SD. (D) Immunoblot showing the levels of TUBGCP6 48 h after siRNA-mediated depletion of WBP11, PRPF8, or SLU7. (E) Quantification of centriole number in mitotic cells after siRNA-mediated knockdown of WBP11, PRPF8, or SLU7 for 48 h. FLAG-TUBGCP6 transgene was induced with doxycycline where indicated. n = 6, ≥50 cells per experiment. Error bars represent SD. (F) Graph showing the percentage of mitotic cells with three to four centrioles, as shown in Fig. 8 E. Unpaired parametric t test: *, P = 0.0429 (WBP11) and 0.0115 (SLU7). Error bars represent SD.