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. 2019 Dec 18;219(1):e201906076. doi: 10.1083/jcb.201906076

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© 2019 Amick et al.

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Figure S4. — Epitope tagging of the endogenous PQLC2 protein. (A) Sequencing traces from an HEK293FT cell line that has a 2xHA epitope tag inserted into the endogenous PQLC2 gene. Example traces from unaffected and edited alleles are shown. This insertion results in a 2xHA tag at the C-terminus of PQLC2. (B) Summary of sequencing results from this cell line. Results are consistent with one of three copies of PQLC2 being 2xHA-tagged at the C-terminus, while the remaining two copies of the gene are unaffected. (C) The specificity of the anti-HA immunofluorescence signal in PQLC2-2xHA cells is supported by the absence of this signal in parental, non–gene-edited cells. (D) Immunofluorescence images showing the localization of PQLC2-2xHA to lysosomes (endogenous PQLC2-2xHA and LAMP1). Scale bars: 10 µm. Insets are 5.9 µm wide.