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. 2019 Dec 9;219(1):e201902088. doi: 10.1083/jcb.201902088

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© 2019 Boyer et al.

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Figure 5. — TRIM67 interacts and localizes with VASP. (A) Coimmunoprecipitation assays from TRIM9^+/+ or TRIM9^−/− HEK293 cells transfected with GFP-VASP and myc or myc-tagged TRIM67 constructs demonstrate an interaction between TRIM67 and VASP that is independent of TRIM9. (B) Coimmunoprecipitation assays from TRIM67^−/− HEK293 cells transfected with the indicated TRIM67 and VASP constructs, showing requirement for the TRIM67 coiled-coil domain for the TRIM67:VASP interaction. (C) Immunoprecipitation of endogenous TRIM67 from cultured embryonic cortical neurons, showing coprecipitation of endogenous VASP. (D) Colocalization between GFP-VASP and tagRFP-tagged constructs of TRIM67 in murine embryonic cortical neurons. (E) Individual data points and box plots of the colocalization between VASP and TRIM67 (obs) in filopodia compared with Fay-randomized controls (rand). Three experiments per TRIM67 construct. n (filopodia) = 281 T67, 270 ΔCC. ***, P < 0.005. Box plots are minimum, Q1, Q2, Q3, maximum.