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. 2019 Dec 9;219(1):e201902088. doi: 10.1083/jcb.201902088

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© 2019 Boyer et al.

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Figure S3. — All domains of TRIM67 are required to rescue netrin-dependent axon branching. (A) ICC of Trim67^−/− neurons expressing indicated myc-tagged TRIM67 constructs, stained for myc (green), filamentous actin (phalloidin, red) and β-III-tubulin (yellow). (B) Individual data points and box-and-whisker plots showing the data spread in the interquartile range (box) and minimum and maximum (whiskers) of axon branches per 100-µm axon length. Percentage of neurons with unbranched axons is shown below the x-axis. Note that the percentage of unbranched axons causes many conditions to have a median branching value at 0. All statistical comparisons in red are to myc-expressing, untreated neurons. n (cultures) = 3. n (cells) = 169 myc media, 177 myc netrin, 173 TRIM67 media, 166 TRIM67 netrin, 80 LD media, 77 LD netrin, 108 ΔRING media, 111 ΔRING netrin, 113 ΔCC media, 116 ΔCC netrin, 76 NH₂ media, 51 NH₂ netrin. ***, P < 0.005. Box plots are minimum, Q1, Q2, Q3, maximum.