Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 12;219(1):e201901097. doi: 10.1083/jcb.201901097

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — CuSO₄ and OsO₄ enhance ionization in their vicinity by a matrix effect. (A) HeLa cell transfected with Mito-APEX2, fixed with OsO₄, and imaged in adjacent ultrathin sections by EM and MIMS. Location of line scan is indicated in second image. Image is representative of 11 Mito-APEX2 transfected HeLa cells (in three technical/two biological replicates). (B) HeLa cell transfected with Mito-APEX2, stained with CuSO₄ (in the absence of OsO₄), and imaged in adjacent ultrathin sections by EM and MIMS. Location of line scan indicated in second image. Image is representative of three Mito-APEX2 transfected HeLa cells (in one technical/biological replicate). (C and D) Average intensity of ions from mitochondrial ROIs compared with random ROIs from the cytosol for samples fixed with OsO₄ (C) or stained with CuSO₄ in the absence of OsO₄ (D). 14 mitochondria from one representative cell in A and B were analyzed in each condition. Dotted lines indicate 100% of cytosol intensity. Error bars represent SD. Scale bar in all images = 1 µm.