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. 2019 Dec 24;219(1):e201907060. doi: 10.1083/jcb.201907060

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© 2019 Greenan et al.

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Figure S2. — Comparing microtubule linkers. We compared the linkers of the proximal centriole with the proximal BB (top panels) and the proximal BB with the TZ (bottom panels). (A) Average of the proximal CHO centriole (EMD-7776; Greenan et al., 2018), with arrows indicating the location of the A-C linker. (B) In the proximal bovine cilium (EMD-20681), a canonical A-C linker is not seen, but densities are observed binding in the A08/A09 area. (C) In the overlay, the common features between the centriole and BB can be appreciated. Note in particular the absence of the A-C linker attaching to protofilaments of the C-tubule in the bovine BB, suggesting a different type of linker. (D) The major densities binding to the outside of the A tubule of the proximal BB (EMD-20681) bind to protofilaments A07 and A08. (E) In the TZ (EMD-20685), the TZ-linker binds predominantly to protofilament A07, with additional contacts with protofilament A06 also seen at a different contour level. Scale bar is 25 nm.