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Imaging subresolution vesicle fluorescence. Live-cell confocal microscopy of cells expressing GFP (left) or GFP-TPD54 (center); GFP-TPD54 knock-in cells are shown to the right. Captured at 120 ms per frame. Playback, 10 frames per second. ROI is 3.5 µm × 3.5 µm, expansion is fivefold.
