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Maximum-intensity 2D projections of 3D image stacks calculated for each 1-min time point. Time given in minutes. Scale bar = 1 µm; 12 fps. Temperature increase begins at 1 min, reaching 42°C at ∼5 min. Coordinated movement of transgene (green) and nuclear speckle (light green) suggesting stable physical attachment of transgene with speckle during HS (see also Fig. S3 F). Red, MS2-tagged nascent transcripts.
