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. 2019 Nov 18;219(1):e201904091. doi: 10.1083/jcb.201904091

Figure 5.

Figure 5.

Cro1p promotes elevated ciliary force-induced SF elongation. (A) Representative images of WT, cro1Δ, and cro1Δ rescue cells during steady-state (25°C) and elevated force-induced state (39°C for 24 h). More cro1Δ cells exhibit local BB disorientation (white arrowhead) and twisted BB rows compared with WT cells at elevated force-induced state. The percentage of each phenotype is calculated based on cro1Δ cells that exhibit BB disorientation. All small insets show a representative BB and SF. Scale bars, 10 µm (cell), 1.3 µm (inset width), 2 µm (BB rows inset). (B) Quantification of BB orientation (R value) of WT, cro1Δ, and cro1Δ rescue cells. cro1Δ cells exhibit BB disorientation at elevated force-induced state. (C) Quantification of SF length of WT, cro1Δ, and cro1Δ rescue cells. SF length of cro1Δ cells failed to elongate at the elevated force-induced state. Arrowhead and dotted line mark the minimal SF length that is required for SF–pcMT interactions. n ≥ 350 SFs (≥40 cells). Mann-Whitney test. * denotes P value <0.01. Mean ± SD. (D) Model depicting how SF base proteins, DisAp and Cro1p, influence SF length. Loss-of-function of these proteins either affect primary (SF–pcMT) or secondary (SF–cell cortex) interactions, and lead to varying degrees of BB disorganization and disorientation. A and D: BB and epiplasm, red; SF, green; pcMTs, cyan; linkages, magenta.