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. Author manuscript; available in PMC: 2020 Feb 24.
Published in final edited form as: Immunol Invest. 2018 Nov;47(8):844–854. doi: 10.1080/08820139.2018.1552360

Figure 3. MDSC can directly differentiate into osteoclasts.

Figure 3.

MDSC (CD11b+ LY6G− LY6Chigh) cell populations were isolated using magnetic cell separation from 12-week-old C57BL/6J mouse spleen and bone marrow and plate for osteoclast differentiation using RANKL/M-CSF to induce osteoclast maturation for 5 days. Note that CD markers for defining MDSC may also represent other monocytic populations and not exclusively MDSCs. Representative TRAP (tartrate resistant acid phosphatase) stained osteoclasts are shown in the left panels. Osteoclasts were enumerated using TRAP+ with ≥3 nuclei and presented in the right panel. (N=3; ***P<0.001).