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. Author manuscript; available in PMC: 2020 Feb 24.
Published in final edited form as: Nat Immunol. 2019 Jun 17;20(8):1046–1058. doi: 10.1038/s41590-019-0414-1

Figure 3.

Figure 3.

Development of Padi492–105-specific tTreg cells is restricted to the neonatal thymus. (a, b) Flow cytometry of Vα2+ CD4+CD8− thymocytes in (a) YAe62β.Foxp3-GFP and (b) Padi4−/−.YAe62β.Foxp3-GFP mice at different ages; numbers adjacent to outlined areas indicate percent IAb-Padi4 tetramer+ Foxp3-GFP− (left) and Foxp3-GFP+ (right). (c-f) Frequency and total cell numbers of IAb-Padi4+ (c, d) CD4+CD8− Foxp3− and (e, f) Foxp3-GFP+ tTreg cells in WT and Padi4−/− YAe62β.Foxp3-GFP mice at different ages. Bars represent the data mean, n = 6, 12, 6, 6, 6, 6 WT mice at 1, 2, 3, 4, and 8 weeks old, n = 7 Padi4−/− 2 and 6 weeks old. (c-f) NS P>0.05, **P<0.01, ***P<0.01, ****P<0.0001 one-way ANOVA Tukey multiple comparisons test.