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. Author manuscript; available in PMC: 2020 Feb 24.
Published in final edited form as: Cell Rep. 2020 Feb 11;30(6):1690–1701.e4. doi: 10.1016/j.celrep.2020.01.021

Figure 5. AGO4 Is an Essential Antiviral Mediator In Vivo.

Figure 5.

(A) Weight loss over time following intranasal infection with influenza A (PR/8/1934, 105 plaque-forming units [pfu]/mouse, n = 10 per group).

(B) Hematoxylin and eosin staining of lungs 4 days p.i. Scale bar represents 0.2 μm.

(C) Proportion of monocyte and dendritic cell populations infiltrating lungs 4 days p.i., as assessed by flow cytometry.

(D) Confocal microscopy of influenza-GFP (green) or wheat germ agglutinin (blue) of lungs 72 h p.i. (105 pfu/mouse). Scale bar represents 350 μm.

(E–H) Viral titers of bronchoalveolar lavage fluid (BALF) measured by plaque assay (E), immunoblot of influenza NP protein (F), or influenza PA (G) or NP (H) RNA expression in lungs measured by qPCR 4 days p.i.

*p < 0.05, **p < 0.01, and ***p < 0.001, as determined by an unpaired t test.