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. Author manuscript; available in PMC: 2020 Jun 4.
Published in final edited form as: Nature. 2019 Dec 4;576(7786):301–305. doi: 10.1038/s41586-019-1814-y

Figure 1. Endogenous redox state in an age-synchronized population of C. elegans larvae.

Figure 1.

(a) Distribution of Grx1-roGFP2 ratios of a L2-staged N2jrIs2[Prpl-17::Grx1-roGFP2] population. L2 worms with Grx1-roGFP2 ratios between 2 and 3 standard deviations above (red line, insert: R2, red, L2ox) or below (blue line, insert: R4, blue, L2red) the mean were sorted and compared to animals with mean Grx1-roGFP2 ratios (green line, insert: R3, green, L2mean). Insert: axes are redrawn to scale. n = 15,599 animals. (b) Representative microscopic analysis of the Grx1-roGFP2 ratio of individual worms (symbols) of the L2ox, L2mean and L2red subpopulations. n = animals; One-way ANOVA with Tukey correction. The experiment was repeated 4 more times with similar results (see Extended Data Figure 2a–d). (c) Longitudinal analysis of the redox state. L2ox and L2 red-sorted were cultivated at 20°C and the Grx1-roGFP2 ratio of animals (symbol) in each subpopulation was determined microscopically at the indicated time points. n = animals; Mann-Whitney U test, two - sided. Data in (b) and (c) represent mean ± SEM.