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. Author manuscript; available in PMC: 2020 Jun 4.
Published in final edited form as: Nature. 2019 Dec 4;576(7786):301–305. doi: 10.1038/s41586-019-1814-y

Extended Data Figure 1. In vivo read-out of endogenous redox states at different stages during C. elegans lifespan and sorting parameters of oxidized and reduced subpopulations.

Extended Data Figure 1.

(a) Microscopic analysis of the Grx1-roGFP2 ratio of individual N2jrIs2[Prpl-17::Grx1-roGFP2] worms (symbol) cultivated at 15 °C and imaged at the indicated time points. Means (bars) of every two sequential time points that are not significantly different from each other (P > 0.05) share the same letter. Data represent mean ± SEM; n, number of animals; one-way ANOVA with Tukey correction. (b) The roGFP2 ratio (405/488) was calculated using the partial profiling feature (pp) configured to analyze extinction and emission data from each 488 nm and 405 nm lasers that sequentially excited each worm. (c) A population of N2jrIs2[Prpl-17::Grx1-roGFP2] at the L2 stage separated based on their opacity (extinction) and length (time of flight) was gated as R1.