Figure 1.

The Nogo-A C-terminal region is secreted in culture supernatant as an exosome component. A, culture supernatants (culture sup) collected from vector- and Nogo-A–Myc–transfected HEK293T cells were immunoprecipitated (IP) with anti-Myc antibody and immunoblotted with anti-Myc antibody. Cell lysates were also immunoprecipitated and immunoblotted in the same membrane. B, schematic of exosome fractionation by centrifugation. C, each fraction from vector or Nogo-A–Myc–transfected HEK293T cells was immunoblotted with anti-Myc antibody. D, ratio of 24-kDa Nogo-A fragment to full-length (FL) Nogo-A in exosome and lysate fractions. Mean ± S.E., n = 12 for each group. ***, p < 0.005; Student's two-tailed t test.