Figure 3.

Treatment with 8a causes decreased viability and decreased phosphorylation of histone H3 (Ser-10) in TNBC cells. A, MDA-MB-231 (left) and MDA-MB-468 (right) cells were treated with DMSO or 8a at concentrations ranging from 1 nm to 30 μm. Cells were exposed to 8a for 72 h before viability was measured using a resazurin assay. Three biological replicates were completed for each cell line, and representative dose–response curves are displayed here. B, MDA-MB-231 and MDA-MB-468 cells were treated with DMSO or 8a at the indicated concentrations. The medium was changed, and fresh inhibitor was added every 2–3 days. Cells were monitored until nearly confluent in the DMSO condition (about 8 or 14 days, respectively), at which point they were stained with crystal violet and imaged. Three (MDA-MB-231) or two (MDA-MB-468) biological replicates were completed, and representative images are shown here. C, asynchronous MDA-MB-468 cells were treated with DMSO or 0.5, 1, or 3 μm 8a for 1, 6, or 24 h. Cells were harvested and immunoblotted with the indicated antibodies. Data displayed here are representative of three biological replicates.