Figure 5.

C-terminal Src kinase interacts with activated LAIR-1 via a phosphorylated Tyr-251 in LAIR-1. Jurkat cells expressing either an empty control, WT human LAIR-1, or tyrosine mutants of LAIR-1 Jurkat cells expressing either an empty control, WT human LAIR-1, or a Tyr-to-Phe point mutation at aa 251, aa 281, or both of LAIR-1 were activated by α1(II) for 5 min. The presence of LAIR-1 and Csk in the resulting immunoprecipitates (IP) were examined by Western blotting analysis using anti–LAIR-1 antibody and anti-Csk antibody, respectively. The phosphorylation patterns of LAIR-1 activation of these mutants is demonstrated in Fig. 4B. The data shown are representative of three separate analyses. IB, immunoblot.