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Inhibition of MMP10 cleavage of endogenous substrates. A, H3 was serially diluted into wells containing a constant concentration of MMP10. Samples were then incubated with AAT to observe whether H3 prevents MMP10-mediated cleavage of AAT. Samples were analyzed by reducing SDS-PAGE. Data are representative of three separate experiments. B, NE activity was measured after the addition of H3/MMP10/AAT samples. Error bars. S.D.; n = 3. RFU, relative fluorescence units.
