Figure 3.

Antibody-dependent cellular cytotoxicity (ADCC) (a) and antibody-dependent cellular phagocytosis (ADCP) (b) of the antibodies against CTLA-4. (a) Human CTLA4-expressing 293F cells were added to 96-well plates at 1 × 10⁴ cells/well, and then the antibodies pre-incubated with 5 × 10⁵ PBMCs were added. The plates were kept at 37°C in a 5% CO₂ incubator for 4 h. Lysis of the target cells was determined by the introduction of DELFIA® EuTDA Cytotoxicity Reagents. (b) Human macrophage cells were mixed at 1:1 ratio with CFSE-dyed engineered human CTLA-4 expressing 293F cells in 96-well plates, then antibodies were added and incubated with cells at 37°C in a 5% CO₂ incubator for 3 h. After wash, APC-labeled anti-human CD14 antibody was added for flow cytometry detection.